Acetaminophen and hepatotoxicity. Acetaminophen (paracetamol) is Download Scientific Diagram Glutathione Depletion in Mitochondrial Diseases Glutathione Reporter Combination of Paracetamol and the Glutathione Depleting Agent Buthionine Sulfoximine Show Differential Effect on Liver Cancer Cells and Normal Hepatocytes View of Acetaminophen, the missing anion gap in the patient with metabolic acidosis The Southwest journal of medicine Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.